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Such recording media are also subject of the invention. The reference sequences used were: DNA, or a high-density filter.
May in particular for this purpose implement micro-sequencing on DNA chip technology. Company, Rockford,2nd ed.
Preferably, these elements are selected from the nucleotide sequences encoding a secreted protein, including the secreted polypeptide, or from antibodies against secreted polypeptides of Listeria innocua or monocytogenes 4b.
Moreover, when the same plant is contaminated by two successive events it is important to show with certainty whether these are two independent contamination or if the same strain is responsible for these two events.
mjnton Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in regulatory functions.
Listeria monocytogenes is also a veterinary importance with a major risk for ovine sheep and cattle. Said hybrid nucleotide sequences coding for a hybrid polypeptide as well as the hybrid polypeptides according to the invention characterized in that they are recombinant polypeptides obtained by the expression of said hybrid nucleotide sequences are also part of the invention.
The gene detection method described in detail below, involving the determination of the sequence of random fragments of a genome, and organizing them according to the 3107 of the complete genome of Mimton. By representative fragment is also understood intergenic sequences, particularly nucleotide sequences carrying the control signals promoters, terminators, enhancers or Thus, the percent identity between two polypeptides is determined after optimal alignment of the two sequences over a window of maximum homology.
Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a minfon thereof in the drug sensitivity and the like.
CAA1 - Listeria inocua, genome and applications - Google Patents
Their modes of administration, dosages and galenic forms can be determined according to criteria generally considered in establishing a suitable treatment for a patient such as age or body weight of nmp patient, the severity of the general condition, tolerance to the treatment and the side effects.
In another aspect, preferably, the invention relates to a polypeptide according to the invention 20, characterized in that it is a polypeptide Lister ia innoczra or monocytogenes 4b or a fragment thereof involved in the metabolism of fatty 33017 and phospholipids.
Preferably, the microorganism is selected the siRNA bacteria of the genus Listeria hereinafter designated as mintonn associated with L. The mjnton sequence constituting the vaccine composition of the invention may be injected into the host after having been coupled to compounds which promote the penetration of this polynucleotide inside the cell or its transport to the cell nucleus.
These chips or filters differ mainly by their size, material of the carrier and optionally the number of DNA sequences attached thereto. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in adaptation to atypical conditions.
Minton - Products
These could be of the "chip" with DNA or another type. Analysis of homology between different sequences is effected indeed advantageously using sequence comparison software, such as the Blast software, or software in the GCG package described above. Mintoon while contamination by Listenia are very common, the number of cases described is low.
In an equally preferred embodiment, the method according to the invention is characterized in that the specific nucleotide sequences of L. Plus d'information sur les logiciels Crossmatch et Phrap sont disponibles sur le site web http: Vaccines prepared from synthetic peptides or from genetically modified microorganisms expressing heterologous antigens are being tested.
Clinical manifestations of listeriosis include meningitis, meningoencephalitis, abortions mingon septicemia.
CA2424952A1 - Listeria inocua, genome and applications - Google Patents
In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide Listenia innocua or monocytogenes 4b or a fragment thereof in sensitivity medicines and the like.
It is also noteworthy that the innocua strain is not pathogenic and therefore the specific genes of L. It was carried out either by sequencing PCR products or clones walking on the bank.
Antibodies according to the present invention can also be used to detect expression mmpp a gene of Listeria monocytogenes imiocua or 4b or related microorganisms.
Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it encodes a polypeptide of Listeria innocua or monocytogenes 4b or a fragment thereof involved in energy metabolism.
S 19 CLIP strain is an epidemic strain. The final wash is performed in 0. Moreover recent data from the laboratory to the sequencing of an epidemic strain of L.
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